PCR has been used to examine oysters for the presence of
Chapter 9, Problem 1(choose chapter or problem)
PCR has been used to examine oysters for the presence of Vibrio cholerae. Oysters from different areas were homogenized, and DNA was extracted from the homogenates. Te DNA was digested by the restriction enzyme HincII. A primer for the hemolysin gene of V. cholerae was used for the PCR reaction. Afer PCR, each sample was electrophoresed and stained with a probe for the hemolysin gene. Which of the oyster samples were (was) positive for V. cholerae? How can you tell? Why look for V. cholerae in oysters? What is the advantage of PCR over conventional biochemical tests to identify the bacteria?
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