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Answer: Treating a particular polypeptide with one reagent (as described in the previous

Chapter 19, Problem 86P

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QUESTION:

Problem 86P

Treating a particular polypeptide with one reagent (as described in the previous problem) produced the following fragments:

asp-thr-ala-trp, gly-glu-ser-lys, trp-arg

Treating the same polypeptide with a different reagent produced the following fragments:

thr-ala-trp, gly-glu, ser-lys-trp-arg-asp

What is the amino acid sequence of the polypeptide?

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QUESTION:

Problem 86P

Treating a particular polypeptide with one reagent (as described in the previous problem) produced the following fragments:

asp-thr-ala-trp, gly-glu-ser-lys, trp-arg

Treating the same polypeptide with a different reagent produced the following fragments:

thr-ala-trp, gly-glu, ser-lys-trp-arg-asp

What is the amino acid sequence of the polypeptide?

ANSWER:

Solution 86P :

Step 1:

The fragments resulting from the first treatment of polypeptide with reagent is :

asp-thr-ala-trp,  gly-glu-ser-lys,  trp-arg

The fragments resulting from the treatment of same polypeptide with different reagent is :

thr-ala-trp , gly-glu , ser-lys-trp-arg-asp

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