The article “Efficient Pyruvate Production by a Multi-Vitamin Auxotroph of Torulopsis glabrata: Key Role and Optimization of Vitamin Levels” (Y. Li, J. Chen, et al. Applied Microbiology and Biotechnology, 2001,:680-685) investigates the effects of the levels of several vitamins in a cell culture on the yield (in g/L) of pyruvate, a useful organic acid. The data in the following table are presented as two replicates of a 2' design. The factors arc A: nicotinic acid. B: thiamine, and C: biotin. (Two statistically insignificant factors have been dropped. In the article, each factor was tested at four levels; we have collapsed these to two.)

A |
B |
C |
Yields |
Mean Yield |

1 |
-1 |
-1 |
0.55,0.49 |
0.520 |

1 |
-1 |
-1 |
0.60,0.42 |
0.510 |

-1 |
1 |
-1 |
0.37,0.28 |
0.325 |

1 |
1 |
-1 |
0.30,0.28 |
0.290 |

-1 |
-1 |
1 |
0.54,0.54 |
0.540 |

1 |
-1 |
1 |
0.54,0.47 |
0.505 |

-l |
1 |
1 |
0.44,0.33 |
0.385 |

1 |
1 |
1 |
0.36,0.20 |
0.280 |

a. Compute estimates of the main effects and interactions, and the sum of squares and P-value for each.

b. Is the additive model appropriate?

c. What conclusions about the factors can be drawn from these results?

MICROBIOLOGY [BIOL 2300] NOTES WEEK OF JAN 10 AND WEEK OF JAN 17 CHAPTER 1 HUMANS AND THE MICROBIAL WORLD OTHER IMPORTANT MED TERMS Normal flora is the human microbiome. Objective: data through test Another name for this is...