Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 106 for the aqueous reaction TRIS TRISH a. What is the optimal pH for TRIS buffers? b. Calculate the ratio [TRIS][TRISH] at pH 7.00 and at pH 9.00. c. A buffer is prepared by diluting 50.0 g TRIS base and 65.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? What is the pH after 0.50 mL of 1
Week 3: Day 7: Review for exam 1; no notes. Day 8: Day of exam 1; no notes. Day 9: REMEMBER THIS RULES: ● ate 3 oxygens. ● it 2 oxygens. ● fate/phat: 4 oxygens. ● CO 2 →o vertical line in letters so 2. ● C2H O 1 →vertical line in letters so 1. ○ Exception: Phosphorus and Ammonium. ○ Phosphide → P. ○ Phosphate → PO43. ○ Phosphite → PO 3. Dihydrogen Phosphate: H2PO4(3) 1 → This is now turned into 1 because you have to add the 3 charge with the 2+, which in conclusion equals 1. Ammonium Sulfate: Put In Water: AQ:
