The Kjeldahl method is used in agricultural chemistry to

Chapter 5, Problem 96

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The Kjeldahl method is used in agricultural chemistry to determine the percent protein in natural products. The method is based on converting all the protein nitrogen to ammonia and then determining the amount of ammonia by titration. The percent nitrogen in the sample under analysis can be calculated from the quantity of ammonia produced. Interestingly, the majority of protein molecules in living matter contain just about 16% nitrogen. A 1.250 g sample of meat is heated with concentrated sulfuric acid and a catalyst to convert all the nitrogen in the meat to Then excess NaOH(aq) is added to the mixture, which is heated to expel All the nitrogen from the sample is found in the which is then absorbed in and neutralized by 50.00 mL of dilute The excess requires 32.24 mL of 0.4498 M NaOH for its titration. Aseparate 25.00 mL sample of H2SO41aq2 H2SO41aq2. NH31g2, NH31g2. 1NH422SO4. Mn2+ + H2O + CO2 1not balanced2 H2C2O4 + MnO4 - + H+ Mn2+ + H2O + CO2 1not balanced2 H2C2O4 + MnO2 + H+ MnO2 the dilute requires 22.24 mL of 0.4498 M NaOH for its titration. What is the percent protein in the meat?