In 3241, give an example of a function or functions with the specified properties. Express your answers using equations, and specify the independent and dependent variables. Five data points for which the average rates of change between consecutive points are positive and the sequential points are increasing at a decreasing rate

Week12(Ithink) TESTMONDAY WEDNESDAY Microplasmas–bacteriawithoutcellwallswithsmallgenomes,485genes Wantedtoputgenomeofoneorganismintoanothertomakeitthefirstone Endonucleaseusedtocut5’endofsinglestrandsoyoucananneal2chromosome partstogethertobuildchromosomefragments Yeastcombinedthemintoacircle Transformrelatedspecies(looklikeeyeballs)membraneandcytoplasmofone organismwithacompletelyartificiallysynthesizedgenome Determineifthegeneswereessential,non-essential,orquasi-essential Hadtoreturnsomenon-essentialgenesbecausethecellwouldnotgrow Theythinkthereneededtoberedundancyoffunction Sometheycoulddeletethattheythoughtwereessential Putgenesinto4groupsoffunction(cytosolicmetabolism,cellmembrane, preservationofgenomeinformation,andexpressionofgenomeinformation [Largest]) NOTTALKINGABOUTRNA FRIDAY Guestlecture HowtoderiveGMOs Humanshave25,000genes.“Wehavethegenomesequenced”=don’tknowexactly whattheydo,blueprintinworkingdocument 20chromosomesofproteins • Recombinationattelomere • GlycinemaxfromGlycinesoja–tookalotofworktogetridofnonneeded genes • Teosinte–cornismoredifferentthanhumansandchimps VariationatUNL • Conventionalhas57000genesandroundupreadyhas57001genes • Planthybridsandputmaleandfemalechromintosisterchromatidstoget recombination • Promotertellsgenewhere,when,andhowmuchtoproducegene • IntegrationeventXregeneration=functionaltransformant • Planttissueculture–cangothroughsomaticembryogenesis(monocots)or organogenesis(formshootorroot,thenflipit,dicots) • Planttransformation– o Microprojectilebombardment–mostcommon o Agrobacterium-mediatedtransformation–bacteriacellformsextreme growth § Abunchofproteinsinvolved(took10labs20yearstofigure out) § Chemotaxis–helpsignalsfromplantandmovestowardthe higherconcentration § ThisactivatesagenetomaketDNAthatmovesthroughthe pore § Resultsincrowngall o Say“heyletstakeoutthesebadgenesandputinthegeneswewantin theplantandletitreplicateandsendittotheplantcells!” o CutandpastegenesintobinaryvectorsinsteadofintegratingintoTi plasmid. • Hairyroots–likeasweetpotato,naturalGMO • Bolistics–byastrawberrybreeder o Nobiologicalprocess,justshootitinlikeashotgun Crossblackflowerandblackandwhiteandgetallwhite–endogenousgenes Zincfinger–makeupdependsonwherewillbind. • Makegeneediting Agrobacteriumuse4proteinpathway Bacteria–sitonneedle3proteinandwhattheyinsertistranscriptionactors. • Effectorsthatactastranscriptionfactorshavemanylittleparts. CRISPR–adaptiveimmunesystem Zincfinger Talons Cas