The isoelectric point of an amino acid is the pH at which the molecule has no net
Chapter 22, Problem 22.167(choose chapter or problem)
The isoelectric point of an amino acid is the pH at which the molecule has no net charge. For glycine, that point would be the pH at which virtually all glycine molecules are in the form 1H3NCH2CO2 2. This form of glycine is amphoteric since it can act as both an acid and a base. If we assume that the principal equilibrium at the isoelectric point has the best acid reacting with the best base present, then the reaction is 21H3NCH2CO2 2mH2NCH2CO2 2 1 1H3NCH2CO2H (i) Assuming this reaction is the principal equilibrium, then the following relationship must hold true: 3H2NCH2CO2 2 4 5 3 1H3NCH2CO2H4 (ii) Use this result and your answer to part c of Exercise 136 to calculate the pH at which equation (ii) is true. This pH will be the isoelectric point of glycine.
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