U Doxorubicin (DOX) is a widely used anthracycline that has been effective intreatments

Chapter 30, Problem 30-12

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U Doxorubicin (DOX) is a widely used anthracycline that has been effective intreatments of leukemia and breast cancer in humans (A. B. Anderson, C. M.Ciriaks, K. M. Fuller, and E. A. Ariaga, Anal. Chern., 2003, 75,8). Unfortunately,side effects, such as liver toxicity and drug resistance, have been reported.In a recent study, Anderson et al. used laser-induced fluorescence (LIF) as a detectionmode for CE to investigate metabolites of DOX in single cells and subcellularfractions. The following are results similar to those obtained by Andersonet al. for quantifying doxorubicin by LIF. The CE peak areas were measuredas a function of the DOX concentration to construct a calibration curve.0.101.005.0010.0020.0030.0050.000.100.804.528.3215.726.241.5(a) Find the equation for the calibration curve and the standard deviations ofthe slope and intercept. Find the R' value.(b) Rearrange the equation found in part (a) to express concentration in termsof the measured area.(c) The limit of detection (LOD) for DOX was found to be 3 X \0-11 M. If theinjection volume was 100 pL, what was the LOD in moles?(d) Two samples of unknown DOX concentration were injected and peak areasof 11.3 and 6.97 obtained. What were the concentrations and their standarddeviations?(e) Under certain conditions, the DOX peak required 300 s to reach the LlFdetector. What time would be required if the applied voltage were doubled?What time would be required if the capillary length were doubled at thesame applied voltage?(f) The capillary used in part (e) under normal conditions had a plate count of100,000. What would N be if the capillary length were doubled at the sameapplied voltage? What would N be if the applied voltage were doubled atthe original capillary length'!(g) For a 40.6-cm-long capillary of inside diameter 50 flm, what would the plateheight be for a capillary with N = !OO,OOO'!(h) For the same capillary as in part (g), what is the variance a' of a typical peak')