A 1O.0-gsample of protein was hydrolyzed. A 3.0-mg portion of I'C-labeledthreonine, with
Chapter 32, Problem 32-16(choose chapter or problem)
A 1O.0-gsample of protein was hydrolyzed. A 3.0-mg portion of I'C-labeledthreonine, with specific activity of 1000 cpm/mg, was added to the hydrolysate.After mixing, 11.5 mg of pure threonine was isolated and found to have a specificactivity of 20 cpm/mg.(a) What percentage of this protein is threonine?(b) Had llC (e1l2 = 20.3 min) been used instead of I'C, how would the answerchange?Assume all numerical values are the same as in part (a) and that the elapsedtime between' the two specific activity measurements was 32 min.
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