Which carbon oxide does not exist?
Bio 97 (#61860): Lecture 13 Class Notes 10/3/2016 (Powerpoint slides “Bacterial Genetics I”) I. Bacterial Clones A. once you spread cells out -> get visible colonies B. colonies result of division C. replicate by binary ﬁssion D. genetic clones of one another II. Mutations in Bacteria A. carbon source mutant - inability to grow when particular sugar is sole sugar or carbon source on medium B. sugars 1. carbons, hydrogens, and oxygens in 1:2:1 ratio a) these are usually sugars 2. polysaccharides need to get broken down into simple sugars 3. needs extra membrane proteins to get into cell 4. cell needs to make extra proteins to utilize these carbohydrate sources C. media 1. MM = glucose (minimal medium) 2. MM + ^x carbon = not glucose a) unless it says AND glucose, assume there are no glucose molecules and whatever is listed is sole carbon source 3. RM = glucose / all metabolites a) glucose and usually has all amino acids and nucleotides D. metabolite mutants 1. chemical that cell utilizes in order for cell to grow 2. anything else that cell needs to grow than carbon source 3. prototrophic a) capable of making everything they need so they grow on minimal medium 4. auxotrophic a) one that has mutation in one of these genes that codes enzyme for producing one or more of these amino acids E. essential amino acids 1. amino ac