Proof that an imine was formed between aldolase and its
Chapter 23, Problem 43(choose chapter or problem)
Proof that an imine was formed between aldolase and its substrate was obtained by using d -fructose -1,6-bisphosphate labeled at the C-2 position with 14 C as the substrate. NaBH 4 was added to the reaction mixture. A radioactive product was isolated from the reaction mixture and hydrolyzed in an acidic solution. Draw the structure of the radioactive product obtained from the acidic solution. ( Hint: NaBH 4 reduces an imine linkage.)
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