Proof that an imine was formed between aldolase and its

Chapter 23, Problem 43

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Proof that an imine was formed between aldolase and its substrate was obtained by using d -fructose -1,6-bisphosphate labeled at the C-2 position with 14 C as the substrate. NaBH 4 was added to the reaction mixture. A radioactive product was isolated from the reaction mixture and hydrolyzed in an acidic solution. Draw the structure of the radioactive product obtained from the acidic solution. ( Hint: NaBH 4 reduces an imine linkage.)

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