- 28.28-1: List the types of substances to which each of the following chromat...
- 28.28-2: Describe three general methods for improving resolution in partitio...
- 28.28-3: Describe a way to manipulate the retention factor of a solute in pa...
- 28.28-4: How can the selectivity factor be manipulated in (a) gas chromatogr...
- 28.28-5: In preparing a hexane-acetone gradient for an alumina HPLC column, ...
- 28.28-6: 6 What is meant by the linear-response range of a detector?
- 28.28-7: Define(a) isocratic elution.(b) gradient elution.(c) stop-flow inje...
- 28.28-8: What is a guard column in partition chromatography?
- 28.28-9: In what way are normal-phase partition chromatography and adsorptio...
- 28.28-10: What is the order in which thc following compounds would be eluted ...
- 28.28-11: What is the order of elution of the following compounds from a norm...
- 28.28-12: Describe the fundamental difference between adsorption and partitio...
- 28.28-13: Describe the fundamental difference between ion-exchange and size-e...
- 28.28-14: What types of species can be separated by HPLC but not by GC"?
- 28.28-15: Describe the various kinds of pumps used in HPLC. What are the adva...
- 28.28-16: Describe the differences between single-column and suppressor-colum...
- 28.28-17: Mass spectrometry is an extremely versatile detection system for ga...
- 28.28-18: Which of the GC detectors in Table 27-1 are suitable for HPLC? Why ...
- 28.28-19: Although temperature does not have nearly the effect on HPLC separa...
- 28.28-20: Two components in an HPLC separation have retention times that diff...
- 28.28-21: An HPLC method was developed for the separation and determination o...
- 28.28-22: [n a normal-phase partition column, a solute was found to have a re...
- 28.28-23: 3 Assume for simplicity that the HPLC plate height, H, can be given...
Solutions for Chapter 28: Liquid Chromatography
Full solutions for Principles of Instrumental Analysis | 6th Edition
An unsaturated hydrocarbon that contains one or more carbon-carbon triple bonds.
A bond to a chair conformation of cyclohexane that extends from the ring parallel to the imaginary axis through the center of the ring; a bond that lies roughly perpendicular to the equator of the ring.
A compound that delivers the elements of a carbene without actually producing a free carbene.
The potential difference between the cathode and anode in an electrochemical cell; it is measured in volts: 1 V = 1 J>C. Also called electromotive force. (Section 20.4)
A model of reaction rates based on the idea that molecules must collide to react; it explains the factors influencing reaction rates in terms of the frequency of collisions, the number of collisions with energies exceeding the activation energy, and the probability that the collisions occur with suitable orientations. (Section 14.5)
A method for selectively cleaving and identifying the N-terminal amino acid of a polypeptide chain.
The process of separating compounds on the basis of their electric charge
A reaction in which the Gibbs free energy of the products is lower than that of the reactants. The position of equilibrium for an exergonic reaction favors products.
A cyclic ester.
lambda max (lmax)
In UVVis spectroscopy, the wavelength of maximum absorption.
A spectrum that contains radiation at only certain specific wavelengths. (Section 6.3)
Molecular orbital (MO) theory
A theory of chemical bonding in which electrons in molecules occupy molecular orbitals that extend over the entire molecule and are formed by the combination of the atomic orbitals that make up the molecule
One of the four arrow-pushing patterns for ionic reactions.
The study of carbon-containing compounds, typically containing carbon–carbon bonds. (Section 2.9; Chapter 24:Introduction)
Small molecules that are trapped between polymer chains where they function as lubricants, preventing the polymer from being brittle.
For light, the orientation of the electric field.
Primary structure of nucleic acids
The sequence of bases along the pentose-phosphodiester backbone of a DNA or RNA molecule read from the 5’ end to the 3’ end
The structure that arises when a protein consists of two or more folded polypeptide chains that aggregate to form one protein complex.
sigma (s) bond
A bond that is characterized by circular symmetry with respect to the bond axis.
The threedimensional shape of a protein.