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Determine the amino acid sequence of a polypeptide from the following data
Chapter 22, Problem 41(choose chapter or problem)
Determine the amino acid sequence of a polypeptide from the following data: Acid-catalyzed hydrolysis gives Ala, Arg, His, 2 Lys, Leu, 2 Met, Pro, 2 Ser, Thr, and Val. Carboxypeptidase A releases Val. Edman’s reagent releases PTH-Leu. Treatment with cyanogen bromide gives three peptides with the following amino acid compositions:
1. His, Lys, Met, Pro, Ser
2. Thr, Val
3. Ala, Arg, Leu, Lys, Met, Ser
Trypsin-catalyzed hydrolysis gives three peptides and a single amino acid:
1. Arg, Leu, Ser
2. Met, Pro, Ser, Thr, Val
3. Lys
4. Ala, His, Lys, Met
Questions & Answers
QUESTION:
Determine the amino acid sequence of a polypeptide from the following data: Acid-catalyzed hydrolysis gives Ala, Arg, His, 2 Lys, Leu, 2 Met, Pro, 2 Ser, Thr, and Val. Carboxypeptidase A releases Val. Edman’s reagent releases PTH-Leu. Treatment with cyanogen bromide gives three peptides with the following amino acid compositions:
1. His, Lys, Met, Pro, Ser
2. Thr, Val
3. Ala, Arg, Leu, Lys, Met, Ser
Trypsin-catalyzed hydrolysis gives three peptides and a single amino acid:
1. Arg, Leu, Ser
2. Met, Pro, Ser, Thr, Val
3. Lys
4. Ala, His, Lys, Met
ANSWER:Step 1 of 4
The sequence of amino acids in a protein is of paramount importance in determining its structure and function.
Each amino acid has unique chemical properties, and their arrangement dictates the protein's three-dimensional shape. This structure, in turn, influences the protein's ability to interact with other molecules and perform its biological role. Even a minor alteration in the amino acid sequence can lead to a non-functional or dysfunctional protein, emphasizing the critical role of the sequence.