A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 17-10. It required 163 L of 1.43 mM ferric nitrilotriacetate to reach the end point. (a) Why does the slope of the absorbance-versus-volume graph change abruptly at the equivalence point? (b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point? (c) Each apotransferrin molecule binds two ferric ions. Find the concentration of apotransferrin in the 2.00-mL solution.
2016/08/04 GENERAL EXPRESSIONS FOR BUFFER This is the expression used to calculate the concentration of the hydronium ion,3[H O ] from the concentration of the weak acid, its conjugatebase and its K . AQUEOUS EQUILIBRIA . a Using the above expression: - + CH 3O H 2 (aq)+