The wavelengths of maximum absorption and emission of anthracene in Figure 17-22 are
Chapter 17, Problem 17-29(choose chapter or problem)
The wavelengths of maximum absorption and emission of anthracene in Figure 17-22 are approximately 357 and 402 nm. Molar absorptivities at these wavelengths are 9.0103 M1 cm1 and 5 101 M1 cm1 . Consider a fluorescence experiment in Figure 17-21 with cell dimensions b1 0.30 cm, b2 0.40 cm, and b3 0.5 cm. Calculate the relative fluorescence intensity with Equation 17-12 as a function of concentration over the range 108 to 103 M. Explain the shape of the curve. Up to approximately what concentration is fluorescence proportional to concentration (within 5%)? Is the calibration range in Figure 17-23 sensible?
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