(b) Electrophoresis is run with the injection end positive and the detection end
Chapter 25, Problem 25-34(choose chapter or problem)
(b) Electrophoresis is run with the injection end positive and the detection end negative. At pH 8.5, both anions have a charge of 2. The electroosmotic flow from the positive terminal to the negative terminal is greater than the electrophoretic flow, so these two anions have a net migration from the positive to the negative end of the capillary in electrophoresis. From your answer to part (a), predict the order of elution of these two species. (c) At pH 4.0, both anions have a charge close to 1, and the electroosmotic flow is weak. Therefore, electrophoresis is run with the injection end negative and the detection end positive. The anions migrate from the negative end of the capillary to the positive end. Predict the order of elution.
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