Entamoeba histolytica and Giardia lamblia were isolated

Chapter 7, Problem 1

(choose chapter or problem)

Entamoeba histolytica and Giardia lamblia were isolated from the stool sample of a 45-year-old man, and Shigella sonnei was isolated from the stool sample of an 18-year-old woman. Both patients experienced diarrhea and severe abdominal cramps, and prior to onset of digestive symptoms both had been treated by the same chiropractor. e chiropractor had administered colonic irrigations (enemas) to these patients. e device used for this treatment was a gravity-dependent apparatus using 12 liters of tap water. ere were no check valves to prevent backow, so all parts of the apparatus could have become contaminated with feces during each colonic treatment. e chiropractor provided colonic treatment to four or ve patients per day. Between patients, the adaptor piece that is inserted into the rectum was placed in a hot-water sterilizer.What two errors were made by the chiropractor?