Answer: Tris(hydroxymethyl)aminomethane, commonly called

Chapter 15, Problem 15.105

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Tris(hydroxymethyl)aminomethane, commonly called TRIS or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and Kb is 1.19 3 1026 for the aqueous reaction 1HOCH22 3CNH2 1 H2Om1HOCH22 3CNH3 1 1 OH2 TRIS TRISH1 a. What is the optimal pH for TRIS buffers? b. Calculate the ratio [TRIS]y[TRISH1] at pH 5 7.00 and at pH 5 9.00. c. A buffer is prepared by diluting 50.0 g TRIS base and 65.0 g TRIS hydrochloride (written as TRISHCl) to a total volume of 2.0 L. What is the pH of this buffer? What is the pH after 0.50 mL of 12 M HCl is added to a 200.0-mL portion of the buffer?

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