Normal hatchery processes in aquaculture inevitably producestress in fish, which may

Chapter 12, Problem 85

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Normal hatchery processes in aquaculture inevitably producestress in fish, which may negatively impact growth,reproduction, flesh quality, and susceptibility to disease.Such stress manifests itself in elevated and sustainedcorticosteroid levels. The article Evaluation of SimpleInstru ments for the Measurement of Blood Glucoseand Lactate, and Plasma Protein as Stress Indicatorsin Fish (J. of the World Aquaculture Society, 1999:276284) described an experiment in which fish weresubjected to a stress protocol and then removed and testedat various times after the protocol had been applied. Theaccompanying data on x 5 time (min) and y 5 bloodglucose level (mmol/L) was read from a plot.x 2 2 5 7 12 13 17 18 23 24 26 28y 4.0 3.6 3.7 4.0 3.8 4.0 5.1 3.9 4.4 4.3 4.3 4.4x 29 30 34 36 40 41 44 56 56 57 60 60y 5.8 4.3 5.5 5.6 5.1 5.7 6.1 5.1 5.9 6.8 4.9 5.7Use the methods developed in this chapter to analyzethe data, and write a brief report summarizing your conclusions(assume that the investigators are particularlyinterested in glucose level 30 min after stress).

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