Why does skeletal muscle look striated?
REVIEW FOR FINAL EXAM-MICRO 2042 LABS-Fall 2017 USE AS GUIDELINE- NOT INCLUSIVE! Read lab manual (answer questions), Read SWI student handbook, TA notes, know how to interpret all results (positive/negative) SWI project 1- Soil collection/culture media- section 2- page 21-24 a. What is important about collection of soil b. Why did we use the type of media that was of low nutrients i. Water agar, Rhizobium, TSA 50% 2- Soil-dilutions a. How did we isolate soil organisms i. Serial dilutions performed (1:10 1:100). why are dilutions needed ii. Calculation of bacterial count, CFU/ml iii. Scientific notation (1x10 or 1,000,000, 000) iv. Viable cell Number (VCN = # colonies X 1/dilution factor) know how to calculate 1. Dilution factor in tubes, plates 2. Know how to calculate viable cell number in X sample 3- The use of the micropipetters (including units) Refer to p.77, 78 Microorganisms Lab Manual a. Volumes in ml and ul. Conversions 4- Isolating soil organisms a. Culture conditions: temperature. Why was it important to incubate at 30oC b. Which criteria did you use to pick colonies from your plates c. Colony morphology p. 34 Microorganisms Lab Manual d. How did you test and assessed production of antibiotics by isolates e. What is antagonism What is antagonistic interaction How did you detect it f.