Crystallins in Mouse Eyes: Investigating Gene Expression in Embryo Dev

Chapter 15, Problem 8

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QUESTION:

Imagine you want to study one of the mouse crystallins, proteins present in the lens of the eye. Assuming the gene has been cloned, describe two ways you could investigate expression of this gene in the developing mouse embryo.

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QUESTION:

Imagine you want to study one of the mouse crystallins, proteins present in the lens of the eye. Assuming the gene has been cloned, describe two ways you could investigate expression of this gene in the developing mouse embryo.

ANSWER:

Step 1 of 3

Cloning of genes refers to the biotechnical process in which any specific gene or gene sequences are duplicated in a laboratory. This in-vitro duplication is done with the aim of studying the gene thoroughly. It is beneficial in understanding the gene's functionality and its other characteristics, such as size.

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