You are studying the binding of proteins to the cytoplasmic face of cultured
Chapter 0, Problem 10-8(choose chapter or problem)
You are studying the binding of proteins to the cytoplasmic face of cultured neuroblastoma cells and have found a method that gives a good yield of inside-out vesicles from the plasma membrane. Unfortunately, your preparations are contaminated with variable amounts of right-side-out vesicles. Nothing you have tried avoids this problem. A friend suggests that you pass your vesicles over an affinity column made of lectin coupled to solid beads. What is the point of your friends suggestion?
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