Molecular Biology of the Cell 6th Edition solutions

Molecular Biology of the Cell 6
Because the DNA double helix is only 2 nm wide well below the limit of resolution of the light microscope it is impossible to see chromosomes in living cells without special stains.

Molecular Biology of the Cell 6
A fluorescent molecule, having absorbed a single photon of light at one wavelength, always emits it at a longer wavelength.

Molecular Biology of the Cell 6
The diagrams in Figure Q91 show the paths of light rays passing through a specimen with a dry lens and with an oil-immersion lens. Offer an explanation for why oil-immersion lenses should give better resolution. Air, glass, and oil have refractive indices of 1.00, 1.51, and 1.51, respectively

Molecular Biology of the Cell 6
Figure Q92 shows a diagram of the human eye. The refractive indices of the components in the light path are: cornea 1.38, aqueous humor 1.33, crystalline lens 1.41, and vitreous humor 1.38. Where does the main refractionthe main focusingoccur? What role do you suppose the lens plays?

Molecular Biology of the Cell 6
Why do humans see so poorly under water? And why do goggles help?

Molecular Biology of the Cell 6
Explain the difference between resolution and magnification.

Molecular Biology of the Cell 6
Antibodies that bind to specific proteins are important tools for defining the locations of molecules in cells. The sensitivity of the primary antibodythe antibody that reacts with the target moleculeis often enhanced by using labeled secondary antibodies that bind to it. What are the advantages and

Molecular Biology of the Cell 6
Figure Q93 shows a series of modified fluorescent proteins that emit light in a range of colors. How do you suppose the exact same chromophore can fluoresce at so many different wavelengths?

Molecular Biology of the Cell 6
Consider a fluorescent detector designed to report the cellular location of active protein tyrosine kinases. A blue (cyan) fluorescent protein (CFP) and a yellow fluorescent protein (YFP) were fused to either end of a hybrid protein domain. The hybrid protein segment consisted of a substrate peptide