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Quantitative Chemical Analysis | 8th Edition | ISBN: 9781429218153 | Authors: Daniel C. Harris
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(a) A chromatography column with a length of 10.3 cm and inner diameter of 4.61 mm is

Chapter 22, Problem 22-19

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QUESTION:

(a) A chromatography column with a length of 10.3 cm and inner diameter of 4.61 mm is packed with a stationary phase that occupies 61.0% of the volume. If the volume flow rate is 1.13 mL/min, find the linear flow rate in cm/min. (b) How long does it take for solvent (which is the same as unretained solute) to pass through the column? (c) Find the retention time for a solute with a retention factor of 10.0.

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QUESTION:

(a) A chromatography column with a length of 10.3 cm and inner diameter of 4.61 mm is packed with a stationary phase that occupies 61.0% of the volume. If the volume flow rate is 1.13 mL/min, find the linear flow rate in cm/min. (b) How long does it take for solvent (which is the same as unretained solute) to pass through the column? (c) Find the retention time for a solute with a retention factor of 10.0.

ANSWER:

Step 1 of 4

(a)

From the given,

Inner diameter = 4.61 nm =0.461 cm

Length of the column = 10.3 cm

The stationary phase = 61%

Mobile phase = (100- 61) = 39%

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