The weight of proteins or nucleic acids in solution is commonly determined by UV
Chapter 20, Problem 20.25(choose chapter or problem)
The weight of proteins or nucleic acids in solution is commonly determined by UV spectros-copy using the Beer-Lambert law. For example, the e of double-stranded DNA at 260 nmis 6670 M21cm21. The formula weight of the repeating unit in DNA (650 Daltons onaverage) can be used as the molecular weight. What is the weight of DNA in 2.0 mL ofaqueous buffer if the absorbance, measured in a 1-cm cuvette, is 0.75?
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