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The weight of proteins or nucleic acids in solution is commonly determined by UV

Organic Chemistry | 7th Edition | ISBN: 9781133952848 | Authors: William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote ISBN: 9781133952848 483

Solution for problem 20.25 Chapter 20

Organic Chemistry | 7th Edition

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Organic Chemistry | 7th Edition | ISBN: 9781133952848 | Authors: William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Organic Chemistry | 7th Edition

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Problem 20.25

The weight of proteins or nucleic acids in solution is commonly determined by UV spectros-copy using the Beer-Lambert law. For example, the e of double-stranded DNA at 260 nmis 6670 M21cm21. The formula weight of the repeating unit in DNA (650 Daltons onaverage) can be used as the molecular weight. What is the weight of DNA in 2.0 mL ofaqueous buffer if the absorbance, measured in a 1-cm cuvette, is 0.75?

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BN Chapter 2: Atoms, Molecules, and Ions 2.1: The Early History of Chemistry  Greeks first to try to explain chemical changes (400BC) o All matter composed of 4 elements:  Fire, earth, water, and air o Matter  Matter is continuous (infinitely divisible into smaller pieces) OR  Composed of small indivisible particles o Democritus  Atomos=atoms  Supported small indivisible particles o No definite answer b/c no experiments  Alchemy (next 2000 yrs) o Mystics obsessed w/ idea of turning cheap metal into gold o Discovery:  How to prepare mineral acids  Elements: mercury, sulfur, and antimony  Robert Boyle, the first "chemist" (1600's) o Measured the relationship b/t pressure & the volume of gases o Element=substance that cannot be broken down further  Combustion (17th-18th cent.) o Georg Stahl: phlogiston flowed out of burning material--stopped burning b/c container filled w/ phlogiston  Phlogiston: substance believed in the 18th cent. that exist in all combustible bodies; released in combustion o Joseph Priestley: vigorous combustion, low in phlogiston o Priestley's observations did not contradict Stahl's--diff. theories for WHY 2.2: Fundamental Chemical Law

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Chapter 20, Problem 20.25 is Solved
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Textbook: Organic Chemistry
Edition: 7
Author: William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
ISBN: 9781133952848

This textbook survival guide was created for the textbook: Organic Chemistry, edition: 7. The answer to “The weight of proteins or nucleic acids in solution is commonly determined by UV spectros-copy using the Beer-Lambert law. For example, the e of double-stranded DNA at 260 nmis 6670 M21cm21. The formula weight of the repeating unit in DNA (650 Daltons onaverage) can be used as the molecular weight. What is the weight of DNA in 2.0 mL ofaqueous buffer if the absorbance, measured in a 1-cm cuvette, is 0.75?” is broken down into a number of easy to follow steps, and 71 words. Since the solution to 20.25 from 20 chapter was answered, more than 259 students have viewed the full step-by-step answer. This full solution covers the following key subjects: . This expansive textbook survival guide covers 24 chapters, and 1131 solutions. Organic Chemistry was written by and is associated to the ISBN: 9781133952848. The full step-by-step solution to problem: 20.25 from chapter: 20 was answered by , our top Chemistry solution expert on 03/16/18, 05:02PM.

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The weight of proteins or nucleic acids in solution is commonly determined by UV