For the binomial queue:a. Modify the merge routine to terminate merging if there are no

Cell Bio  DNA restriction Enzymes  DNA modification enzymes o DNA polymerase o Polynucleotide Kinase o DNA ligase (glue)  Fig 8-24 o Restriction endonuclease o Let Dhruv into mortar board  Fig 8-25 o Electrophoresis o Migration top to bottom - negative to positive o Based on size of fragment o Agarose gel o Big to small molecular weight o E10-1 o Reverse compliment Dna sequencing  Panel 8-1 o dNTP: 3' OH o DdNTP is chain terminating and blocks elongation o Diagrams are important in this panel o NEED primer o In the gel the short one is on the bottom  Read it bottom to top : means it's upstream towards the 5' end  TEST ON THIS o Florescence marking with diagram  Fully automated o E10-2  Illumina sequencing (SBS)  Sequencing biosynthesis  Most popular out of all of them  Taking lots of pictures when nucleotides are being aided o Shot gun sequencing  Can cut DNA into specific size  Problem when you hit repetitive sequence (~50% is) o Clone by clone- more specific  BAC clones  Whole genome into larger fragments o Gene se