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A compound X is to be determined by UV-visible spectrophotometry. A c.alibrationcurve is
Chapter 13, Problem 13-26(choose chapter or problem)
A compound X is to be determined by UV-visible spectrophotometry. A c.alibrationcurve is constructed from standard solutions of X with the following. results:0.50 ppm, A = 0.24; 1.5 ppm, A = 0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 'o~9;4.5 ppm, A = 0.70. A solution of unknown X concentration had an absorbanceof A = 0.50. Find the slope and intercept of the calibration curve, the standarderror in Y, the concentration of the solution of unknown X concentration, andthe standard deviation in the concentration of X. Construct a plot of the calibrationcurve and determine the unknown concentration by hand from the plot.Compare it to that obtained from the regression line.
Questions & Answers
QUESTION:
A compound X is to be determined by UV-visible spectrophotometry. A c.alibrationcurve is constructed from standard solutions of X with the following. results:0.50 ppm, A = 0.24; 1.5 ppm, A = 0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 'o~9;4.5 ppm, A = 0.70. A solution of unknown X concentration had an absorbanceof A = 0.50. Find the slope and intercept of the calibration curve, the standarderror in Y, the concentration of the solution of unknown X concentration, andthe standard deviation in the concentration of X. Construct a plot of the calibrationcurve and determine the unknown concentration by hand from the plot.Compare it to that obtained from the regression line.
ANSWER:Problem 13-26
A compound X is to be determined by UV-visible spectrophotometry. A calibration curve is constructed from standard solutions of X with the following results: 0.50 ppm, A = 0.24; 1.5 ppm, A = 0.36; 2.5 ppm, A = 0.44; 3.5 ppm, A = 0.59; 4.5 ppm, A = 0.70. A solution of unknown X concentration had an absorbance of A = 0.50. Find the slope and intercept of the calibration curve, the standard error in Y, the concentration of the solution of unknown X concentration, andthe standard deviation in the concentration of X. Construct a plot of the calibration curve and determine the unknown concentration by hand from the plot.Compare it to that obtained from the regression line.
Step by step solution
Step 1 of 4
From the given,
The following results obtained from the standard solutions are listed below.
Let’s draw a collaboration curve for the given concentration and absorbance value.
The curve is shown below.