The form of the Scatchard equation given in Impact I5.2 applies only when the

Chapter 5, Problem 5.27

(choose chapter or problem)

The form of the Scatchard equation given in Impact I5.2 applies only when the macromolecule has identical and independent binding sites. For non-identical independent binding sites, the Scatchard equation is

\(\frac{v}{[\mathrm{~A}]_{\text {out }}}=\sum_i \frac{N_i K_i}{1+K_i[\mathrm{~A}]_{out}}\)

Plot \(v /[\mathrm{A}]\) for the following cases.

(a) There are four independent sites on an enzyme molecule and the intrinsic binding constant is \(K=1.0 \times 10^7\).

(b) There are a total of six sites per polymer. Four of the sites are identical and have an intrinsic binding constant of \(1 \times 10^5\). The binding constants for the other two sites are \(2 \times 10^6\).