The melting temperature of a DNA molecule can be determined by differential scanning
Chapter 19, Problem 19.31(choose chapter or problem)
The melting temperature of a DNA molecule can be determined by differential scanning calorimetry (Impact I2.1). The following data were obtained in aqueous solutions containing the specified concentration csalt of an soluble ionic solid for a series of DNA molecules with varying base pair composition, with f the fraction of G-C base pairs: csalt = 1.0 102 mol dm3 f 0.375 0.509 0.589 0.688 0.750 Tm/K 339 344 348 351 354 csalt = 0.15 mol dm3 f 0.375 0.509 0.589 0.688 0.750 Tm/K 359 364 368 371 374 (a) Estimate the melting temperature of a DNA molecule containing 40.0 per cent G-C base pairs in both samples. Hint. Begin by plotting Tm against fraction of G-C base pairs and examining the shape of the curve. (b) Do the data show an effect of concentration of ions in solution on the melting temperature of DNA? If so, provide a molecular interpretation for the effect you observe.
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