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SCIENTIFIC INQUIRY Imagine protein X, destined to span the plasma membrane. Assume that

Chapter 6, Problem 10

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QUESTION:

SCIENTIFIC INQUIRY Imagine protein X, destined to span the plasma membrane. Assume that the mRNA carrying the genetic message for protein X has already been translated by ribosomes in a cell culture. If you fractionate the cells (see Figure 6.4), in which fraction would you find protein X? Explain by describing its transit through the cell.

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QUESTION:

SCIENTIFIC INQUIRY Imagine protein X, destined to span the plasma membrane. Assume that the mRNA carrying the genetic message for protein X has already been translated by ribosomes in a cell culture. If you fractionate the cells (see Figure 6.4), in which fraction would you find protein X? Explain by describing its transit through the cell.

ANSWER:


Protein X would be found in the Outer Membrane Fraction (OMF) since it is a membrane-spanning protein. It would have traversed through the cytoplasm, Endoplasmic Reticulum (ER), Golgi Apparatus,

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