The following data were taken from a diode-array spectrophotometer in an experimentto

Chapter 13, Problem 13-22

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QUESTION:

The following data were taken from a diode-array spectrophotometer in an experimentto measure the spectrum of the Co(II)-EDTA complex. The columnlabeled P;o'"",," is the relative signal obtained with sample solution in the cellafter subtraction of the dark signal. The column labeled Pm",,,, is the referencesignal obtained with only solvent in the cell after subtraction of the dark signal.Find the transmittance at each wavelength. and the absorbance at each wavelength.Plot the spectrum of th.: compound.

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QUESTION:

The following data were taken from a diode-array spectrophotometer in an experimentto measure the spectrum of the Co(II)-EDTA complex. The columnlabeled P;o'"",," is the relative signal obtained with sample solution in the cellafter subtraction of the dark signal. The column labeled Pm",,,, is the referencesignal obtained with only solvent in the cell after subtraction of the dark signal.Find the transmittance at each wavelength. and the absorbance at each wavelength.Plot the spectrum of th.: compound.

ANSWER:

Step 1 of 7

To determine the transmittance following formula is used,

And to determine absorbance following formula is used,

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