Light-induced degradation of molecules, also called photobleaching, is a serious problem
Chapter 14, Problem 14.12(choose chapter or problem)
Light-induced degradation of molecules, also called photobleaching, is a serious problem in single-molecule spectroscopy. A molecule of a fluorescent dye commonly used to label biopolymers can withstand about 106 excitations by photons before light-induced reactions destroy its system and the molecule no longer fluoresces. For how long will a single dye molecule fluoresce while being excited by 1.0 mW of 488 nm radiation from a continuous-wave argon ion laser? You may assume that the dye has an absorption spectrum that peaks at 488 nm and that every photon delivered by the laser is absorbed by the molecule.
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