In Problems 122, sketch the graph of each function. Do not use a graphing calculator. (Assume the largest possible domain.) y = x2 + 1
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Textbook Solutions for Calculus For Biology and Medicine (Calculus for Life Sciences Series)
Question
MichaelisMenten Equation Enzymes serve as catalysts in many chemical reactions in living systems. The simplest such reactions transform a single substrate into a product with the help of an enzyme. The MichaelisMenten equation describes the initial velocity of such enzymatically controlled reactions. The equation, which gives the relationship between the initial velocity of the reaction (v0) and the concentration of the substrate (s0), is v0 = vmaxs0 s0 + Km where vmax is the maximum velocity at which the product may be formed and Km is the MichaelisMenten constant. Note that this equation has the same form as the Monod growth function. (a) Show that the MichaelisMenten equation can be written in the form 1 v0 = Km vmax 1 s0 + 1 vmax This formula is known as the LineweaverBurk equation and shows that there is a linear relationship between 1/v0 and 1/s0. (b) Sketch the graph of the LineweaverBurk equation. Use a coordinate system in which 1/s0 is on the horizontal axis and 1/v0 is on the vertical axis. Show that the resulting graph is a line that intersects the horizontal axis at 1/Km and the vertical axis at 1/vmax. (c) To determine Km and vmax, we measure the initial velocity of the reaction, denoted by v0, as a function of the concentration of the substrate, denoted by s0, and fit a straight line through the points in a coordinate system in which the horizontal axis is 1/s0 and the vertical axis is 1/v0. Explain how to determine Km and vmax from the graph. (Note that this is an example in which a nonlogarithmic transformation is used to obtain a linear relationship. Since the reciprocals of both quantities of interest are used, the resulting plot is called a double-reciprocal plot.)
Solution
The first step in solving 1.3 problem number 86 trying to solve the problem we have to refer to the textbook question: MichaelisMenten Equation Enzymes serve as catalysts in many chemical reactions in living systems. The simplest such reactions transform a single substrate into a product with the help of an enzyme. The MichaelisMenten equation describes the initial velocity of such enzymatically controlled reactions. The equation, which gives the relationship between the initial velocity of the reaction (v0) and the concentration of the substrate (s0), is v0 = vmaxs0 s0 + Km where vmax is the maximum velocity at which the product may be formed and Km is the MichaelisMenten constant. Note that this equation has the same form as the Monod growth function. (a) Show that the MichaelisMenten equation can be written in the form 1 v0 = Km vmax 1 s0 + 1 vmax This formula is known as the LineweaverBurk equation and shows that there is a linear relationship between 1/v0 and 1/s0. (b) Sketch the graph of the LineweaverBurk equation. Use a coordinate system in which 1/s0 is on the horizontal axis and 1/v0 is on the vertical axis. Show that the resulting graph is a line that intersects the horizontal axis at 1/Km and the vertical axis at 1/vmax. (c) To determine Km and vmax, we measure the initial velocity of the reaction, denoted by v0, as a function of the concentration of the substrate, denoted by s0, and fit a straight line through the points in a coordinate system in which the horizontal axis is 1/s0 and the vertical axis is 1/v0. Explain how to determine Km and vmax from the graph. (Note that this is an example in which a nonlogarithmic transformation is used to obtain a linear relationship. Since the reciprocals of both quantities of interest are used, the resulting plot is called a double-reciprocal plot.)
From the textbook chapter Graphing you will find a few key concepts needed to solve this.
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